There are two basic techniques for HLA-typing: Serological HLA-typing (antibody-based typing). Old school! Genomic HLA-typing (PCR-based). PCR-SSP

With LinkSēq, you can achieve high throughput. By using inexpensive thermal cyclers for the first 60 minutes of the amplification step and only 10 minutes on a real time PCR instrument, you can type multiple samples per hour with unmatched simplicity. Products include HLA, KIR, RBC, RH, ABO, and HPA.

The method is based on the principle that only primers whose sequences are perfectly complementary to that of the target sequence of a Aims. Our main aim was to standardize a simple inexpensive in-house PCR-SSP technique for HLA-B* 27 typing. Materials and Methods. Sequence Specific primers were designed to amplify all the subtypes of B* 27 using IMGT-HLA sequence database. Accuracy was checked by retyping of 90 PCR-SSOP typed controls. Results.

• Replicación in vitro. • Basado en HLA-A2 (s). HLA-A *02:01-05. HLA-A *02:01.

Tissue Antigens. 2006;67(3):233-6.

Our main aim was to standardize a simple inexpensive in-house PCR-SSP technique for HLA-B* 27 typing. Materials and Methods. Sequence Specific primers

The distribution frequencies of the HLA-DQB1 gene under different replication states of HBV were compared. 2012-11-30 Get more information about the Olerup SSP HLA typing kits and other products.

PCR with sequence speciﬁc primers (PCR-SSP) One commonly applied approach is to use panels of sequence speciﬁc DNA primers which amplify particular HLA alleles or allele groups. PCR-SSP is a very rapid test that can be performed in 3 – 4 hours and is commonly used for typing deceased organ donors when speed is an important consideration.

HLA-DR typing was performed using standard microcytotoxicity assay and PCR-SSP method in 28 patients referred to our Transplantation Immunology Unit for HLA typing. Comparison of results obtained by both methods revealed no discrepancies in 5 patients, in 12 patients the PCR-SSP typing showed additional DR antigens or splits of antigens. This chapter describes a polymerase chain reaction sequence-specific primer (PCR-SSP) typing system that is applicable to the identification of all HLA class I and class II alleles, as well as alleles from non-HLA loci (10, 11) .

The combination of PCR technology and hybridization with sequence-specific oligonucleotide probes was first applied to HLA class II typing because of the limitations of DR serology and of the better knowledge of allelic polymorphism at DR/DQ loci. 46 Nathalang O, Tantimavanich S, Nillakupt K, Arnutti P, Jaruchaimontree C. HLA-B27 testing in Thai patients using the PCR-SSP technique.
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Human leukocyte antigen (HLA) typing, utilising the sequence-specific oligonucleotide (SSO) and sequence-specific primer (SSP) technologies, has been in routine use in many tissue typing laboratories worldwide for more than 20 years since the development of the polymerase chain reaction. Both methods are very useful for clinical and research The new HLA-Ready Gene kits PCR mastermix (Taq) included.

Sequence-specific amplification (SSP) is simply a form of polymerase chain reaction (PCR) which involves designing one or both primers so that they will or will not allow amplification (the 3'-mismatch principle).
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Get more information about the Olerup SSP HLA typing kits and other products. Back to HLA Kits HLA-A. Product No. Product name No. Of tests Lot List price; 101

HLAアレルのシークエンス配列に併せてプライマーを設計し、その増幅パターンを基にしてタイピングを実施します。. 日本国内においてはOne Lambda社のマイクロSSPやAllSetが市販キットとして利用されております。.